Fully integrated L-phenylalanine separation and concentration using
reactive-extraction with liquid-liquid centrifuges in a fed-batch
process with E-coli
A novel in situ product recovery (ISPR) approach for the (fully)
integrated separation of L-phenylalanine (L-phe) from a 20 l fed-batch
process with the recombinant L-tyrosine auxotrophic strain E. coli
F-4/pF81 is presented. The strain was rationally constructed for the
production of the aromatic amino acid. Glucose and tyrosine control is
used. A reactive extraction system consisting of kerosene, the
cation-selective carrier D(2)EHPA and sulphuric acid, all circulating in
liquid-liquid centrifuges, is applied for the on-line L-phe separation
from cell- and protein-free permeate. Permeate is drained off from the
bioreactor bypass. Using the novel ISPR approach, a significantly
extended product formation period at 0.25 mmol/(g*h) together with a
reduced by-product formation and a 28\% relative glucose/L-phe yield
increase is observed. Thus, the ISPR approach is superior to the
reference non-ISPR process and even offers extraction rates
approximately three times higher than the published membrane-based
process.
%0 Journal Article
%1 ISI:000222368600006
%A Ruffer, N
%A Heidersdorf, U
%A Kretzers, I
%A Sprenger, GA
%A Raeven, L
%A Takors, R
%C 233 SPRING ST, NEW YORK, NY 10013 USA
%D 2004
%I SPRINGER
%J BIOPROCESS AND BIOSYSTEMS ENGINEERING
%K myown
%N 4
%P 239-248
%R 10.1007/s00449-004-0354-4
%T Fully integrated L-phenylalanine separation and concentration using
reactive-extraction with liquid-liquid centrifuges in a fed-batch
process with E-coli
%U https://doi.org/10.1007/s00449-004-0354-4
%V 26
%X A novel in situ product recovery (ISPR) approach for the (fully)
integrated separation of L-phenylalanine (L-phe) from a 20 l fed-batch
process with the recombinant L-tyrosine auxotrophic strain E. coli
F-4/pF81 is presented. The strain was rationally constructed for the
production of the aromatic amino acid. Glucose and tyrosine control is
used. A reactive extraction system consisting of kerosene, the
cation-selective carrier D(2)EHPA and sulphuric acid, all circulating in
liquid-liquid centrifuges, is applied for the on-line L-phe separation
from cell- and protein-free permeate. Permeate is drained off from the
bioreactor bypass. Using the novel ISPR approach, a significantly
extended product formation period at 0.25 mmol/(g*h) together with a
reduced by-product formation and a 28\% relative glucose/L-phe yield
increase is observed. Thus, the ISPR approach is superior to the
reference non-ISPR process and even offers extraction rates
approximately three times higher than the published membrane-based
process.
@article{ISI:000222368600006,
abstract = {{A novel in situ product recovery (ISPR) approach for the (fully)
integrated separation of L-phenylalanine (L-phe) from a 20 l fed-batch
process with the recombinant L-tyrosine auxotrophic strain E. coli
F-4/pF81 is presented. The strain was rationally constructed for the
production of the aromatic amino acid. Glucose and tyrosine control is
used. A reactive extraction system consisting of kerosene, the
cation-selective carrier D(2)EHPA and sulphuric acid, all circulating in
liquid-liquid centrifuges, is applied for the on-line L-phe separation
from cell- and protein-free permeate. Permeate is drained off from the
bioreactor bypass. Using the novel ISPR approach, a significantly
extended product formation period at 0.25 mmol/(g{*}h) together with a
reduced by-product formation and a 28\% relative glucose/L-phe yield
increase is observed. Thus, the ISPR approach is superior to the
reference non-ISPR process and even offers extraction rates
approximately three times higher than the published membrane-based
process.}},
added-at = {2018-06-08T11:31:18.000+0200},
address = {{233 SPRING ST, NEW YORK, NY 10013 USA}},
affiliation = {{Takors, R (Reprint Author), Forschungszentrum Julich, Inst Biotechnol, Postfach 1913, D-52425 Julich, Germany.
Forschungszentrum Julich, Inst Biotechnol, D-52425 Julich, Germany.
Univ Stuttgart, Inst Microbiol, D-70569 Stuttgart, Germany.
DSM Biotech GmbH, D-52428 Julich, Germany.}},
author = {Ruffer, N and Heidersdorf, U and Kretzers, I and Sprenger, GA and Raeven, L and Takors, R},
author-email = {{r.takors@fz-juelich.de}},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/20a74d715f6e550c87ff95cfe042dd64a/ralftakors},
da = {{2018-01-26}},
doc-delivery-number = {{833VN}},
doi = {{10.1007/s00449-004-0354-4}},
interhash = {378f74372aa5fe09a0b256d4edf986de},
intrahash = {0a74d715f6e550c87ff95cfe042dd64a},
issn = {{1615-7591}},
journal = {{BIOPROCESS AND BIOSYSTEMS ENGINEERING}},
journal-iso = {{Bioprocess. Biosyst. Eng.}},
keywords = {myown},
keywords-plus = {{CORYNEBACTERIUM-GLUTAMICUM; AMINO-ACIDS; PILOT-SCALE; FEEDING STRATEGY;
FERMENTATION; GLUCOSE; PHENYLPYRUVATE; OPTIMIZATION; CULTIVATION;
RECOVERY}},
language = {{English}},
month = {{JUL}},
number = {{4}},
number-of-cited-references = {{40}},
orcid-numbers = {{Sprenger, Georg/0000-0002-7879-8978}},
pages = {{239-248}},
publisher = {{SPRINGER}},
research-areas = {{Biotechnology \& Applied Microbiology; Engineering}},
researcherid-numbers = {{Sprenger, Georg/E-2384-2011}},
times-cited = {{36}},
timestamp = {2018-06-08T09:31:18.000+0200},
title = {{Fully integrated L-phenylalanine separation and concentration using
reactive-extraction with liquid-liquid centrifuges in a fed-batch
process with E-coli}},
type = {{Article}},
unique-id = {{ISI:000222368600006}},
url = {https://doi.org/10.1007/s00449-004-0354-4},
usage-count-last-180-days = {{0}},
usage-count-since-2013 = {{8}},
volume = {{26}},
web-of-science-categories = {{Biotechnology \& Applied Microbiology; Engineering, Chemical}},
year = {{2004}}
}
