%0 Journal Article %1 ISI:000397705000011 %A Michalowski, Annette %A Siemann-Herzberg, Martin %A Takors, Ralf %C 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA %D 2017 %I ACADEMIC PRESS INC ELSEVIER SCIENCE %J METABOLIC ENGINEERING %K myown %P 93-103 %R 10.1016/j.ymben.2017.01.005 %T Escherichia coli HGT: Engineered for high glucose throughput even under slowly growing or resting conditions %U https://doi.org/10.1016/j.ymben.2017.01.005 %V 40 %X Aerobic production-scale processes are constrained by the technical limitations of maximum oxygen transfer and heat removal. Consequently, microbial activity is often controlled via limited nutrient feeding to maintain it within technical operability. Here, we present an alternative approach based on a newly engineered Escherichia coli strain. This E. coli HGT (high glucose throughput) strain was engineered by modulating the stringent response regulation program and decreasing the activity of pyruvate dehydrogenase. The strain offers about three-fold higher rates of cell-specific glucose uptake under nitrogen-limitation (0.6 g(Glc) gCDW(-1) h(-1)) compared to that of wild type, with a maximum glucose uptake rate of about 1.8 gGlc gCDW(-1) h(-1) already at a 0.3 h(-1) specific growth rate. The surplus of imported glucose is almost completely available via pyruvate and is used to fuel pyruvate and lactate formation. Thus, E. coli HGT represents a novel chassis as a host for pyruvate-derived products.

@article{ISI:000397705000011, abstract = {{Aerobic production-scale processes are constrained by the technical limitations of maximum oxygen transfer and heat removal. Consequently, microbial activity is often controlled via limited nutrient feeding to maintain it within technical operability. Here, we present an alternative approach based on a newly engineered Escherichia coli strain. This E. coli HGT (high glucose throughput) strain was engineered by modulating the stringent response regulation program and decreasing the activity of pyruvate dehydrogenase. The strain offers about three-fold higher rates of cell-specific glucose uptake under nitrogen-limitation (0.6 g(Glc) gCDW(-1) h(-1)) compared to that of wild type, with a maximum glucose uptake rate of about 1.8 gGlc gCDW(-1) h(-1) already at a 0.3 h(-1) specific growth rate. The surplus of imported glucose is almost completely available via pyruvate and is used to fuel pyruvate and lactate formation. Thus, E. coli HGT represents a novel chassis as a host for pyruvate-derived products.}}, added-at = {2018-06-08T12:10:59.000+0200}, address = {{525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA}}, affiliation = {{Takors, R (Reprint Author), Univ Stuttgart, Inst Biochem Engn, Allmandring 31, D-70569 Stuttgart, Germany. Michalowski, Annette; Siemann-Herzberg, Martin; Takors, Ralf, Univ Stuttgart, Inst Biochem Engn, Allmandring 31, D-70569 Stuttgart, Germany.}}, author = {Michalowski, Annette and Siemann-Herzberg, Martin and Takors, Ralf}, author-email = {{siemann@ibvt.uni-stuttgart.de takors@ibvt.uni-stuttgart.de}}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2a56e3eb3d208bfaa0be132ff5aefc4c1/ralftakors}, da = {{2018-01-26}}, doc-delivery-number = {{EP9PH}}, doi = {{10.1016/j.ymben.2017.01.005}}, eissn = {{1096-7184}}, funding-acknowledgement = {{European Union {[}289326, KBBE.2011.3.6-03]}}, funding-text = {{Special thanks to Michael Kraml and Ulrike Hillemann for their technical support during fermentation processes, sampling procedures and strain constructions. We thank Mira Lenfers-Lucker for her help with HPLC measurements. We are also grateful for the collaboration with Belen Calles, Esteban Martinez and Sofia Fraile (CSIC, CNB, University of Madrid) for providing the pEMG and pACBSR plasmids and appreciate their support with the I-SceI method. The authors would like to thank the European Union for funding these studies as part of the `ST-Flow' project (Grant no.: 289326) in the framework 7 program KBBE.2011.3.6-03.}}, interhash = {28213182eb6e495264220b808312268f}, intrahash = {a56e3eb3d208bfaa0be132ff5aefc4c1}, issn = {{1096-7176}}, journal = {{METABOLIC ENGINEERING}}, journal-iso = {{Metab. Eng.}}, keywords = {myown}, keywords-plus = {{STRINGENT RESPONSE; CARBON METABOLISM; RPOS DEGRADATION; GLYCOLYTIC FLUX; STRESS-RESPONSE; PPGPP SYNTHESIS; PROTEIN; ACID; RELA; TRANSCRIPTION}}, language = {{English}}, month = {{MAR}}, number-of-cited-references = {{45}}, pages = {{93-103}}, publisher = {{ACADEMIC PRESS INC ELSEVIER SCIENCE}}, research-areas = {{Biotechnology \& Applied Microbiology}}, times-cited = {{4}}, timestamp = {2018-06-08T10:10:59.000+0200}, title = {{Escherichia coli HGT: Engineered for high glucose throughput even under slowly growing or resting conditions}}, type = {{Article}}, unique-id = {{ISI:000397705000011}}, url = {https://doi.org/10.1016/j.ymben.2017.01.005}, usage-count-last-180-days = {{5}}, usage-count-since-2013 = {{9}}, volume = {{40}}, web-of-science-categories = {{Biotechnology \& Applied Microbiology}}, year = {{2017}} }