%0 Journal Article %1 Ziegler2011 %A Ziegler, S. %A Eiseler, T. %A Scholz, R.-P. %A Beck, A. %A Link, G. %A Hausser, A. %D 2011 %J Molecular Biology of the Cell %K 2011 hausser izi %N 5 %P 570--580 %R 10.1091/mbc.E10-05-0427 %T A novel protein kinase D phosphorylation site in the tumor suppressor Rab interactor 1 is critical for coordination of cell migration %U http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E10-05-0427 %V 22 %X The multifunctional signal adapter protein Ras and Rab interactor 1 (RIN1) is a Ras effector protein involved in the regulation of epithelial cell processes such as cell migration and endocytosis. RIN1 signals via two downstream pathways, namely the activation of Rab5 and Abl family kinases. Protein kinase D (PKD) phosphorylates RIN1 at serine 351 in vitro, thereby regulating interaction with 14-3-3 proteins. Here, we report the identification of serine 292 in RIN1 as an in vivo PKD phosphorylation site. PKD-mediated phosphorylation at this site was confirmed with a phospho-specific antibody and by mass spectrometry. We demonstrate that phosphorylation at serine 292 controls RIN1-mediated inhibition of cell migration by modulating the activation of Abl kinases. We further provide evidence that RIN1 in vivo phosphorylation at serine 351 occurs independently of PKD. Collectively, our data identify a novel PKD signaling pathway through RIN1 and Abl kinases that is involved in the regulation of actin remodeling and cell migration. %Z Ziegler, SusanneEiseler, TimScholz, Rolf-PeterBeck, AlexanderLink, GiselaHausser, AngelikaengResearch Support, Non-U.S. Gov'tMol Biol Cell. 2011 Mar 1;22(5):570-80. doi: 10.1091/mbc.E10-05-0427. Epub 2011 Jan 5. %7 2011/01/07 %@ 1939-4586 (Electronic)$\backslash$n1059-1524 (Linking)

@article{Ziegler2011, abstract = {The multifunctional signal adapter protein Ras and Rab interactor 1 (RIN1) is a Ras effector protein involved in the regulation of epithelial cell processes such as cell migration and endocytosis. RIN1 signals via two downstream pathways, namely the activation of Rab5 and Abl family kinases. Protein kinase D (PKD) phosphorylates RIN1 at serine 351 in vitro, thereby regulating interaction with 14-3-3 proteins. Here, we report the identification of serine 292 in RIN1 as an in vivo PKD phosphorylation site. PKD-mediated phosphorylation at this site was confirmed with a phospho-specific antibody and by mass spectrometry. We demonstrate that phosphorylation at serine 292 controls RIN1-mediated inhibition of cell migration by modulating the activation of Abl kinases. We further provide evidence that RIN1 in vivo phosphorylation at serine 351 occurs independently of PKD. Collectively, our data identify a novel PKD signaling pathway through RIN1 and Abl kinases that is involved in the regulation of actin remodeling and cell migration.}, added-at = {2023-06-29T13:07:55.000+0200}, annote = {Ziegler, SusanneEiseler, TimScholz, Rolf-PeterBeck, AlexanderLink, GiselaHausser, AngelikaengResearch Support, Non-U.S. Gov'tMol Biol Cell. 2011 Mar 1;22(5):570-80. doi: 10.1091/mbc.E10-05-0427. Epub 2011 Jan 5.}, author = {Ziegler, S. and Eiseler, T. and Scholz, R.-P. and Beck, A. and Link, G. and Hausser, A.}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2b7b46f23342a8502e56e4b49ab73cd6b/fabian}, doi = {10.1091/mbc.E10-05-0427}, edition = {2011/01/07}, interhash = {8d1e7bd28ea0fea95f0da4dd7fcf54da}, intrahash = {b7b46f23342a8502e56e4b49ab73cd6b}, isbn = {1939-4586 (Electronic)$\backslash$n1059-1524 (Linking)}, issn = {1059-1524}, journal = {Molecular Biology of the Cell}, keywords = {2011 hausser izi}, number = 5, pages = {570--580}, pmid = {21209314}, timestamp = {2023-06-29T13:07:55.000+0200}, title = {{A novel protein kinase D phosphorylation site in the tumor suppressor Rab interactor 1 is critical for coordination of cell migration}}, url = {http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E10-05-0427}, volume = 22, year = 2011 }